簡要描述:4T1(小鼠乳腺癌細(xì)胞),來源于小鼠乳腺腺癌,正常的細(xì)胞形態(tài)為上皮樣,貼壁生長。
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品牌 | Life-iLab/李記生物 |
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產(chǎn)品描述
4T1(中文名稱:小鼠乳腺癌細(xì)胞),來源于小鼠乳腺腺癌,正常的細(xì)胞形態(tài)為上皮樣,貼壁生長。
訂購信息
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
4T1 | AX31L184 | T25/1 mL |
運(yùn)輸方式
常溫/干冰運(yùn)輸。
使用方法
培養(yǎng)方法
培養(yǎng)基 | RPMI-1640 90%(Gibco: C11875500BT), Fetal Bovine Serum(GIBCO:10099-141)或和元李記特級(jí)胎牛血清 (Foetal Bovine Serum)(貨號(hào):AC03L055) 10% |
培養(yǎng)條件 | 37℃ 5% CO2 |
消化條件 | 0.25% (w/v) Trypsin-0.53mM EDTA溶液(GIBCO: 25200-056) 或 和元李記0.25%胰蛋的白酶消化液(含EDTA,不含酚紅)(貨號(hào):AC15L821),消化1-3min |
傳代比例 | 1:3-1:5 |
換液時(shí)間 | 2-3天換液一次 |
凍存方法
凍存液 | 55%培養(yǎng)基,40%FBS, 5% (v/v) DMSO或 和元李記快速細(xì)胞凍存液(無血清、無蛋白)(貨號(hào):AC05L033) |
凍存密度 | 1-3 ×106 /管,液氮保存 |
具體操作方法見細(xì)胞使用說明書
注意事項(xiàng)
本產(chǎn)品僅限于科學(xué)實(shí)驗(yàn)研究使用,不得用于臨床診斷、治療等領(lǐng)域。
當(dāng)收到細(xì)胞后,請仔細(xì)閱讀細(xì)胞使用說明書,按規(guī)定操作并記錄細(xì)胞狀態(tài)(拍照)。我司為客戶提供一周內(nèi)的售后保障,特定情況下可免費(fèi)重發(fā)細(xì)胞。
參考文獻(xiàn)
Pulaski BA, et al. Immunotherapy with vaccines combining MHC class II/CD80+ tumor cells with interleukin-12 reduces established metastatic disease and stimulates immune effectors and monokine induced by interferon gamma. Cancer Immunol. Immunother. 49: 34-45, 2000. PubMed: 10782864
Pulaski BA, Ostrand-Rosenberg S. Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res. 58: 1486-1493, 1998. PubMed: 9537252
Pulaski BA, et al. Cooperativity of Staphylococcal aureus enterotoxin B superantigen, major histocompatibility complex class II, and CD80 for immunotherapy of advanced spontaneous metastases in a clinically relevant postoperative mouse breast cancer model. Cancer Res. 60: 2710-2715, 2000. PubMed: 10825145
Aslakson CJ, Miller FR. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res. 52: 1399-1405, 1992. PubMed: 1540948
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特級(jí)胎牛血清 (Foetal Bovine Serum)(貨號(hào):AC03L055)
RPMI-1640 medium (含L-谷氨的酰胺)(貨號(hào):AC01L064)
本產(chǎn)品僅限于科學(xué)實(shí)驗(yàn)研究使用,不得用于臨床診斷、治療等領(lǐng)域。
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